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We already discussed some regulatory issues when we talked about cellular conditions necessary for passage from G1 and G2. Here, we will discuss more specifically the proteins that interact to regulate the cell cycle. The "checkpoints" that we described earlier are established by proteins that use cues from the cell's environment to trigger the entry to and exit from the distinct phases of the cell cycle. We will discuss two main families of proteins involved in this process—cyclin-dependent protein kinases (Cdks) and cyclins.